WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2968114
total bases: 427408416
Q20 bases: 414267305(96.9254%)
Q30 bases: 390675834(91.4057%)

Read1 after filtering:
total reads: 2957711
total bases: 425867102
Q20 bases: 413362088(97.0636%)
Q30 bases: 390017073(91.5819%)

Filtering result:
reads passed filter: 2957711
reads failed due to low quality: 5979
reads failed due to too many N: 4424
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 59.503%

JSON report: PortGambleWA_FG184.json
HTML report: PortGambleWA_FG184.html

fastp -i PortGambleWA_FG184.F.fq.gz -o PortGambleWA_FG184.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG184.json -h PortGambleWA_FG184.html 
fastp v0.20.0, time used: 37 seconds