WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 6581418
total bases: 947724192
Q20 bases: 918776171(96.9455%)
Q30 bases: 866292288(91.4076%)

Read1 after filtering:
total reads: 6558617
total bases: 944387181
Q20 bases: 916802662(97.0791%)
Q30 bases: 864853424(91.5783%)

Filtering result:
reads passed filter: 6558617
reads failed due to low quality: 13330
reads failed due to too many N: 9471
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.4006%

JSON report: PortGambleWA_FG183.json
HTML report: PortGambleWA_FG183.html

fastp -i PortGambleWA_FG183.F.fq.gz -o PortGambleWA_FG183.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG183.json -h PortGambleWA_FG183.html 
fastp v0.20.0, time used: 71 seconds