WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3385780 total bases: 487552320 Q20 bases: 472582783(96.9297%) Q30 bases: 444827063(91.2368%) Read1 after filtering: total reads: 3373760 total bases: 485800080 Q20 bases: 471547991(97.0663%) Q30 bases: 444075786(91.4112%) Filtering result: reads passed filter: 3373760 reads failed due to low quality: 7046 reads failed due to too many N: 4974 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 60.0755% JSON report: PortGambleWA_FG182.json HTML report: PortGambleWA_FG182.html fastp -i PortGambleWA_FG182.F.fq.gz -o PortGambleWA_FG182.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j PortGambleWA_FG182.json -h PortGambleWA_FG182.html fastp v0.20.0, time used: 40 seconds