WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCG

Read1 before filtering:
total reads: 2975929
total bases: 428533776
Q20 bases: 416406121(97.17%)
Q30 bases: 399603890(93.2491%)

Read1 after filtering:
total reads: 2952070
total bases: 425067924
Q20 bases: 413365665(97.247%)
Q30 bases: 396787683(93.3469%)

Filtering result:
reads passed filter: 2952070
reads failed due to low quality: 4323
reads failed due to too many N: 1675
reads failed due to too short: 17861
reads with adapter trimmed: 22098
bases trimmed due to adapters: 2599810

Duplication rate (may be overestimated since this is SE data): 58.9221%

JSON report: MontereyBayCA_Q402.json
HTML report: MontereyBayCA_Q402.html

fastp -i MontereyBayCA_Q402.F.fq.gz -o MontereyBayCA_Q402.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_Q402.json -h MontereyBayCA_Q402.html 
fastp v0.20.0, time used: 29 seconds