WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3269065
total bases: 470745360
Q20 bases: 456766269(97.0304%)
Q30 bases: 430609107(91.4739%)

Read1 after filtering:
total reads: 3258150
total bases: 469128380
Q20 bases: 455808400(97.1607%)
Q30 bases: 429909279(91.64%)

Filtering result:
reads passed filter: 3258150
reads failed due to low quality: 6177
reads failed due to too many N: 4738
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 58.3549%

JSON report: MontereyBayCA_449.json
HTML report: MontereyBayCA_449.html

fastp -i MontereyBayCA_449.F.fq.gz -o MontereyBayCA_449.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_449.json -h MontereyBayCA_449.html 
fastp v0.20.0, time used: 41 seconds