WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4842911
total bases: 697379184
Q20 bases: 675960599(96.9287%)
Q30 bases: 636930588(91.332%)

Read1 after filtering:
total reads: 4825758
total bases: 694843420
Q20 bases: 674488247(97.0705%)
Q30 bases: 635869767(91.5127%)

Filtering result:
reads passed filter: 4825758
reads failed due to low quality: 10260
reads failed due to too many N: 6893
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.6936%

JSON report: MontereyBayCA_448.json
HTML report: MontereyBayCA_448.html

fastp -i MontereyBayCA_448.F.fq.gz -o MontereyBayCA_448.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_448.json -h MontereyBayCA_448.html 
fastp v0.20.0, time used: 54 seconds