WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4709355
total bases: 678147120
Q20 bases: 657632634(96.9749%)
Q30 bases: 619475844(91.3483%)

Read1 after filtering:
total reads: 4693247
total bases: 675760775
Q20 bases: 656233911(97.1104%)
Q30 bases: 618466066(91.5215%)

Filtering result:
reads passed filter: 4693247
reads failed due to low quality: 9337
reads failed due to too many N: 6771
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.0709%

JSON report: MontereyBayCA_447.json
HTML report: MontereyBayCA_447.html

fastp -i MontereyBayCA_447.F.fq.gz -o MontereyBayCA_447.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_447.json -h MontereyBayCA_447.html 
fastp v0.20.0, time used: 53 seconds