WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4285362
total bases: 617092128
Q20 bases: 598137797(96.9284%)
Q30 bases: 563544500(91.3226%)

Read1 after filtering:
total reads: 4270029
total bases: 614857002
Q20 bases: 596825530(97.0674%)
Q30 bases: 562596919(91.5004%)

Filtering result:
reads passed filter: 4270029
reads failed due to low quality: 9118
reads failed due to too many N: 6215
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 61.2097%

JSON report: MontereyBayCA_446.json
HTML report: MontereyBayCA_446.html

fastp -i MontereyBayCA_446.F.fq.gz -o MontereyBayCA_446.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_446.json -h MontereyBayCA_446.html 
fastp v0.20.0, time used: 47 seconds