WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3118519
total bases: 449066736
Q20 bases: 433329127(96.4955%)
Q30 bases: 406116022(90.4356%)

Read1 after filtering:
total reads: 3116280
total bases: 448351861
Q20 bases: 433067937(96.5911%)
Q30 bases: 405984360(90.5504%)

Filtering result:
reads passed filter: 3116280
reads failed due to low quality: 1481
reads failed due to too many N: 758
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 40.1957%

JSON report: MontereyBayCA_439.json
HTML report: MontereyBayCA_439.html

fastp -i MontereyBayCA_439.F.fq.gz -o MontereyBayCA_439.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_439.json -h MontereyBayCA_439.html 
fastp v0.20.0, time used: 40 seconds