WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4607828
total bases: 663527232
Q20 bases: 640758032(96.5685%)
Q30 bases: 600353169(90.4791%)

Read1 after filtering:
total reads: 4604568
total bases: 662292257
Q20 bases: 640328557(96.6837%)
Q30 bases: 600149285(90.617%)

Filtering result:
reads passed filter: 4604568
reads failed due to low quality: 1991
reads failed due to too many N: 1269
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 39.4566%

JSON report: MontereyBayCA_435.json
HTML report: MontereyBayCA_435.html

fastp -i MontereyBayCA_435.F.fq.gz -o MontereyBayCA_435.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_435.json -h MontereyBayCA_435.html 
fastp v0.20.0, time used: 53 seconds