WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2365246
total bases: 340595424
Q20 bases: 330360943(96.9951%)
Q30 bases: 310945573(91.2947%)

Read1 after filtering:
total reads: 2357145
total bases: 339396187
Q20 bases: 329651567(97.1288%)
Q30 bases: 310429073(91.4651%)

Filtering result:
reads passed filter: 2357145
reads failed due to low quality: 4632
reads failed due to too many N: 3469
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.3792%

JSON report: MontereyBayCA_434.json
HTML report: MontereyBayCA_434.html

fastp -i MontereyBayCA_434.F.fq.gz -o MontereyBayCA_434.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_434.json -h MontereyBayCA_434.html 
fastp v0.20.0, time used: 32 seconds