WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4121176
total bases: 593449344
Q20 bases: 575908934(97.0443%)
Q30 bases: 543401287(91.5666%)

Read1 after filtering:
total reads: 4106863
total bases: 591365842
Q20 bases: 574670112(97.1768%)
Q30 bases: 542494964(91.7359%)

Filtering result:
reads passed filter: 4106863
reads failed due to low quality: 8297
reads failed due to too many N: 6016
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 64.0783%

JSON report: MontereyBayCA_434-SEQREP.json
HTML report: MontereyBayCA_434-SEQREP.html

fastp -i MontereyBayCA_434-SEQREP.F.fq.gz -o MontereyBayCA_434-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_434-SEQREP.json -h MontereyBayCA_434-SEQREP.html 
fastp v0.20.0, time used: 47 seconds