WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5328126
total bases: 767250144
Q20 bases: 740783733(96.5505%)
Q30 bases: 696726279(90.8082%)

Read1 after filtering:
total reads: 5309050
total bases: 764452776
Q20 bases: 739142173(96.6891%)
Q30 bases: 695538950(90.9852%)

Filtering result:
reads passed filter: 5309050
reads failed due to low quality: 11298
reads failed due to too many N: 7778
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 64.8859%

JSON report: MontereyBayCA_433-SEQREP.json
HTML report: MontereyBayCA_433-SEQREP.html

fastp -i MontereyBayCA_433-SEQREP.F.fq.gz -o MontereyBayCA_433-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_433-SEQREP.json -h MontereyBayCA_433-SEQREP.html 
fastp v0.20.0, time used: 60 seconds