WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3322921
total bases: 478500624
Q20 bases: 464325118(97.0375%)
Q30 bases: 437567830(91.4456%)

Read1 after filtering:
total reads: 3311723
total bases: 476869142
Q20 bases: 463349170(97.1648%)
Q30 bases: 436851856(91.6083%)

Filtering result:
reads passed filter: 3311723
reads failed due to low quality: 6317
reads failed due to too many N: 4881
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.9386%

JSON report: MontereyBayCA_428.json
HTML report: MontereyBayCA_428.html

fastp -i MontereyBayCA_428.F.fq.gz -o MontereyBayCA_428.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_428.json -h MontereyBayCA_428.html 
fastp v0.20.0, time used: 42 seconds