WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4650591
total bases: 669685104
Q20 bases: 648584309(96.8491%)
Q30 bases: 610893995(91.2211%)

Read1 after filtering:
total reads: 4634162
total bases: 667271052
Q20 bases: 647170665(96.9877%)
Q30 bases: 609874012(91.3982%)

Filtering result:
reads passed filter: 4634162
reads failed due to low quality: 9735
reads failed due to too many N: 6694
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 61.8365%

JSON report: MontereyBayCA_427.json
HTML report: MontereyBayCA_427.html

fastp -i MontereyBayCA_427.F.fq.gz -o MontereyBayCA_427.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_427.json -h MontereyBayCA_427.html 
fastp v0.20.0, time used: 52 seconds