WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3054327
total bases: 439823088
Q20 bases: 426583465(96.9898%)
Q30 bases: 402144853(91.4333%)

Read1 after filtering:
total reads: 3043944
total bases: 438288912
Q20 bases: 425682403(97.1237%)
Q30 bases: 401492316(91.6045%)

Filtering result:
reads passed filter: 3043944
reads failed due to low quality: 6066
reads failed due to too many N: 4317
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 59.5596%

JSON report: MontereyBayCA_426.json
HTML report: MontereyBayCA_426.html

fastp -i MontereyBayCA_426.F.fq.gz -o MontereyBayCA_426.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_426.json -h MontereyBayCA_426.html 
fastp v0.20.0, time used: 41 seconds