WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3358106
total bases: 483567264
Q20 bases: 464506550(96.0583%)
Q30 bases: 434854648(89.9264%)

Read1 after filtering:
total reads: 3321350
total bases: 477870032
Q20 bases: 462426641(96.7683%)
Q30 bases: 433863918(90.7912%)

Filtering result:
reads passed filter: 3321350
reads failed due to low quality: 35806
reads failed due to too many N: 950
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 45.9223%

JSON report: MontereyBayCA_425.json
HTML report: MontereyBayCA_425.html

fastp -i MontereyBayCA_425.F.fq.gz -o MontereyBayCA_425.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_425.json -h MontereyBayCA_425.html 
fastp v0.20.0, time used: 43 seconds