WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2958732
total bases: 426057408
Q20 bases: 412136595(96.7326%)
Q30 bases: 386876268(90.8038%)

Read1 after filtering:
total reads: 2956755
total bases: 425582847
Q20 bases: 411927164(96.7913%)
Q30 bases: 386749250(90.8752%)

Filtering result:
reads passed filter: 2956755
reads failed due to low quality: 1183
reads failed due to too many N: 794
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 46.2107%

JSON report: MontereyBayCA_422.json
HTML report: MontereyBayCA_422.html

fastp -i MontereyBayCA_422.F.fq.gz -o MontereyBayCA_422.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_422.json -h MontereyBayCA_422.html 
fastp v0.20.0, time used: 38 seconds