WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4347050
total bases: 625975200
Q20 bases: 605572472(96.7406%)
Q30 bases: 569986992(91.0558%)

Read1 after filtering:
total reads: 4332742
total bases: 623885194
Q20 bases: 604345956(96.8681%)
Q30 bases: 569101896(91.219%)

Filtering result:
reads passed filter: 4332742
reads failed due to low quality: 8506
reads failed due to too many N: 5802
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 62.7559%

JSON report: MontereyBayCA_421.json
HTML report: MontereyBayCA_421.html

fastp -i MontereyBayCA_421.F.fq.gz -o MontereyBayCA_421.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_421.json -h MontereyBayCA_421.html 
fastp v0.20.0, time used: 49 seconds