WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1538317
total bases: 221517648
Q20 bases: 214610161(96.8817%)
Q30 bases: 201801518(91.0995%)

Read1 after filtering:
total reads: 1532871
total bases: 220723730
Q20 bases: 214141861(97.0181%)
Q30 bases: 201463865(91.2742%)

Filtering result:
reads passed filter: 1532871
reads failed due to low quality: 3215
reads failed due to too many N: 2231
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 62.8173%

JSON report: MontereyBayCA_420.json
HTML report: MontereyBayCA_420.html

fastp -i MontereyBayCA_420.F.fq.gz -o MontereyBayCA_420.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_420.json -h MontereyBayCA_420.html 
fastp v0.20.0, time used: 25 seconds