WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3809331
total bases: 548543664
Q20 bases: 530474263(96.7059%)
Q30 bases: 497683399(90.7281%)

Read1 after filtering:
total reads: 3806538
total bases: 547809356
Q20 bases: 530170190(96.7801%)
Q30 bases: 497510334(90.8182%)

Filtering result:
reads passed filter: 3806538
reads failed due to low quality: 1738
reads failed due to too many N: 1055
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 42.6988%

JSON report: MontereyBayCA_415.json
HTML report: MontereyBayCA_415.html

fastp -i MontereyBayCA_415.F.fq.gz -o MontereyBayCA_415.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_415.json -h MontereyBayCA_415.html 
fastp v0.20.0, time used: 46 seconds