WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3646922
total bases: 525156768
Q20 bases: 508202646(96.7716%)
Q30 bases: 477295402(90.8863%)

Read1 after filtering:
total reads: 3644415
total bases: 524479664
Q20 bases: 507919976(96.8426%)
Q30 bases: 477129793(90.972%)

Filtering result:
reads passed filter: 3644415
reads failed due to low quality: 1454
reads failed due to too many N: 1053
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 46.1528%

JSON report: MontereyBayCA_413.json
HTML report: MontereyBayCA_413.html

fastp -i MontereyBayCA_413.F.fq.gz -o MontereyBayCA_413.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_413.json -h MontereyBayCA_413.html 
fastp v0.20.0, time used: 47 seconds