WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4552076
total bases: 655498944
Q20 bases: 634161665(96.7449%)
Q30 bases: 595370739(90.8271%)

Read1 after filtering:
total reads: 4549009
total bases: 654508121
Q20 bases: 633782538(96.8334%)
Q30 bases: 595167442(90.9335%)

Filtering result:
reads passed filter: 4549009
reads failed due to low quality: 1757
reads failed due to too many N: 1310
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 42.9968%

JSON report: MontereyBayCA_412.json
HTML report: MontereyBayCA_412.html

fastp -i MontereyBayCA_412.F.fq.gz -o MontereyBayCA_412.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_412.json -h MontereyBayCA_412.html 
fastp v0.20.0, time used: 53 seconds