WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2906976
total bases: 418604544
Q20 bases: 404529313(96.6376%)
Q30 bases: 379272298(90.604%)

Read1 after filtering:
total reads: 2904870
total bases: 417929680
Q20 bases: 404272428(96.7322%)
Q30 bases: 379137727(90.7181%)

Filtering result:
reads passed filter: 2904870
reads failed due to low quality: 1248
reads failed due to too many N: 858
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 46.641%

JSON report: MontereyBayCA_409.json
HTML report: MontereyBayCA_409.html

fastp -i MontereyBayCA_409.F.fq.gz -o MontereyBayCA_409.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_409.json -h MontereyBayCA_409.html 
fastp v0.20.0, time used: 38 seconds