WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2309535
total bases: 332573040
Q20 bases: 321230471(96.5895%)
Q30 bases: 301032603(90.5162%)

Read1 after filtering:
total reads: 2307745
total bases: 332052729
Q20 bases: 321026645(96.6794%)
Q30 bases: 300922560(90.6249%)

Filtering result:
reads passed filter: 2307745
reads failed due to low quality: 1135
reads failed due to too many N: 655
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.1044%

JSON report: MontereyBayCA_408.json
HTML report: MontereyBayCA_408.html

fastp -i MontereyBayCA_408.F.fq.gz -o MontereyBayCA_408.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_408.json -h MontereyBayCA_408.html 
fastp v0.20.0, time used: 32 seconds