WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2907593
total bases: 418693392
Q20 bases: 405156153(96.7668%)
Q30 bases: 379855147(90.7239%)

Read1 after filtering:
total reads: 2905429
total bases: 418224319
Q20 bases: 404938448(96.8233%)
Q30 bases: 379718823(90.7931%)

Filtering result:
reads passed filter: 2905429
reads failed due to low quality: 1333
reads failed due to too many N: 831
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 42.5981%

JSON report: MontereyBayCA_407.json
HTML report: MontereyBayCA_407.html

fastp -i MontereyBayCA_407.F.fq.gz -o MontereyBayCA_407.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_407.json -h MontereyBayCA_407.html 
fastp v0.20.0, time used: 38 seconds