WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2845336
total bases: 409728384
Q20 bases: 395762169(96.5913%)
Q30 bases: 370822601(90.5045%)

Read1 after filtering:
total reads: 2843282
total bases: 408981315
Q20 bases: 395498424(96.7033%)
Q30 bases: 370694768(90.6386%)

Filtering result:
reads passed filter: 2843282
reads failed due to low quality: 1275
reads failed due to too many N: 779
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 40.6425%

JSON report: MontereyBayCA_406.json
HTML report: MontereyBayCA_406.html

fastp -i MontereyBayCA_406.F.fq.gz -o MontereyBayCA_406.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_406.json -h MontereyBayCA_406.html 
fastp v0.20.0, time used: 37 seconds