WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3284223
total bases: 472928112
Q20 bases: 457650775(96.7696%)
Q30 bases: 429133636(90.7397%)

Read1 after filtering:
total reads: 3281975
total bases: 472413779
Q20 bases: 457417677(96.8256%)
Q30 bases: 428989700(90.808%)

Filtering result:
reads passed filter: 3281975
reads failed due to low quality: 1345
reads failed due to too many N: 903
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.8532%

JSON report: MontereyBayCA_405.json
HTML report: MontereyBayCA_405.html

fastp -i MontereyBayCA_405.F.fq.gz -o MontereyBayCA_405.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_405.json -h MontereyBayCA_405.html 
fastp v0.20.0, time used: 43 seconds