WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 5499747
total bases: 791963568
Q20 bases: 766830952(96.8265%)
Q30 bases: 719995686(90.9127%)

Read1 after filtering:
total reads: 5496018
total bases: 790762522
Q20 bases: 766371352(96.9155%)
Q30 bases: 719749252(91.0196%)

Filtering result:
reads passed filter: 5496018
reads failed due to low quality: 2156
reads failed due to too many N: 1573
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.8786%

JSON report: MontereyBayCA_404.json
HTML report: MontereyBayCA_404.html

fastp -i MontereyBayCA_404.F.fq.gz -o MontereyBayCA_404.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_404.json -h MontereyBayCA_404.html 
fastp v0.20.0, time used: 61 seconds