WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3061264
total bases: 440822016
Q20 bases: 426324435(96.7112%)
Q30 bases: 399753563(90.6837%)

Read1 after filtering:
total reads: 3059087
total bases: 440348548
Q20 bases: 426102608(96.7648%)
Q30 bases: 399613239(90.7493%)

Filtering result:
reads passed filter: 3059087
reads failed due to low quality: 1286
reads failed due to too many N: 891
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.6508%

JSON report: MontereyBayCA_401.json
HTML report: MontereyBayCA_401.html

fastp -i MontereyBayCA_401.F.fq.gz -o MontereyBayCA_401.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_401.json -h MontereyBayCA_401.html 
fastp v0.20.0, time used: 40 seconds