WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2259430
total bases: 325357920
Q20 bases: 314574957(96.6858%)
Q30 bases: 294806898(90.61%)

Read1 after filtering:
total reads: 2257450
total bases: 324811316
Q20 bases: 314358855(96.782%)
Q30 bases: 294690468(90.7267%)

Filtering result:
reads passed filter: 2257450
reads failed due to low quality: 1301
reads failed due to too many N: 679
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 44.8939%

JSON report: MontereyBayCA_400.json
HTML report: MontereyBayCA_400.html

fastp -i MontereyBayCA_400.F.fq.gz -o MontereyBayCA_400.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_400.json -h MontereyBayCA_400.html 
fastp v0.20.0, time used: 32 seconds