WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... AAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAG Read1 before filtering: total reads: 2902379 total bases: 417942576 Q20 bases: 406435975(97.2468%) Q30 bases: 390513531(93.4371%) Read1 after filtering: total reads: 2878384 total bases: 414422115 Q20 bases: 403347977(97.3278%) Q30 bases: 387657956(93.5418%) Filtering result: reads passed filter: 2878384 reads failed due to low quality: 4306 reads failed due to too many N: 1672 reads failed due to too short: 18017 reads with adapter trimmed: 27484 bases trimmed due to adapters: 2658082 Duplication rate (may be overestimated since this is SE data): 74.5096% JSON report: DabobBayWA_Q358.json HTML report: DabobBayWA_Q358.html fastp -i DabobBayWA_Q358.F.fq.gz -o DabobBayWA_Q358.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q358.json -h DabobBayWA_Q358.html fastp v0.20.0, time used: 29 seconds