WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... ATAGTTGTTATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATC Read1 before filtering: total reads: 2822532 total bases: 406444608 Q20 bases: 395407763(97.2845%) Q30 bases: 379822415(93.45%) Read1 after filtering: total reads: 2789402 total bases: 401630733 Q20 bases: 391009121(97.3554%) Q30 bases: 375669988(93.5362%) Filtering result: reads passed filter: 2789402 reads failed due to low quality: 3968 reads failed due to too many N: 1539 reads failed due to too short: 27623 reads with adapter trimmed: 33577 bases trimmed due to adapters: 4018801 Duplication rate (may be overestimated since this is SE data): 72.9146% JSON report: DabobBayWA_Q243.json HTML report: DabobBayWA_Q243.html fastp -i DabobBayWA_Q243.F.fq.gz -o DabobBayWA_Q243.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q243.json -h DabobBayWA_Q243.html fastp v0.20.0, time used: 27 seconds