WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GTTTTATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCT Read1 before filtering: total reads: 2342278 total bases: 337288032 Q20 bases: 327889822(97.2136%) Q30 bases: 314921420(93.3687%) Read1 after filtering: total reads: 2326070 total bases: 334253702 Q20 bases: 325231502(97.3008%) Q30 bases: 312498151(93.4913%) Filtering result: reads passed filter: 2326070 reads failed due to low quality: 3397 reads failed due to too many N: 1353 reads failed due to too short: 11458 reads with adapter trimmed: 50127 bases trimmed due to adapters: 2348984 Duplication rate (may be overestimated since this is SE data): 74.0322% JSON report: DabobBayWA_Q237.json HTML report: DabobBayWA_Q237.html fastp -i DabobBayWA_Q237.F.fq.gz -o DabobBayWA_Q237.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q237.json -h DabobBayWA_Q237.html fastp v0.20.0, time used: 24 seconds