WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CAGGGTTAGGAACATTAGAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTA Read1 before filtering: total reads: 2946534 total bases: 424300896 Q20 bases: 412057258(97.1144%) Q30 bases: 395282421(93.1609%) Read1 after filtering: total reads: 2927732 total bases: 421565538 Q20 bases: 409786469(97.2059%) Q30 bases: 393230642(93.2786%) Filtering result: reads passed filter: 2927732 reads failed due to low quality: 4883 reads failed due to too many N: 1788 reads failed due to too short: 12131 reads with adapter trimmed: 15964 bases trimmed due to adapters: 1773073 Duplication rate (may be overestimated since this is SE data): 71.7175% JSON report: DabobBayWA_Q236.json HTML report: DabobBayWA_Q236.html fastp -i DabobBayWA_Q236.F.fq.gz -o DabobBayWA_Q236.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q236.json -h DabobBayWA_Q236.html fastp v0.20.0, time used: 31 seconds