WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTA Read1 before filtering: total reads: 3125942 total bases: 450135648 Q20 bases: 436537994(96.9792%) Q30 bases: 418737374(93.0247%) Read1 after filtering: total reads: 3107593 total bases: 447460207 Q20 bases: 434331418(97.0659%) Q30 bases: 416749137(93.1366%) Filtering result: reads passed filter: 3107593 reads failed due to low quality: 4838 reads failed due to too many N: 1988 reads failed due to too short: 11523 reads with adapter trimmed: 16743 bases trimmed due to adapters: 1690764 Duplication rate (may be overestimated since this is SE data): 73.9494% JSON report: DabobBayWA_Q234.json HTML report: DabobBayWA_Q234.html fastp -i DabobBayWA_Q234.F.fq.gz -o DabobBayWA_Q234.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q234.json -h DabobBayWA_Q234.html fastp v0.20.0, time used: 34 seconds