WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... ACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGAATGT Read1 before filtering: total reads: 2350641 total bases: 338492304 Q20 bases: 329026954(97.2037%) Q30 bases: 315971248(93.3467%) Read1 after filtering: total reads: 2329640 total bases: 334302643 Q20 bases: 325192855(97.275%) Q30 bases: 312366102(93.4381%) Filtering result: reads passed filter: 2329640 reads failed due to low quality: 3212 reads failed due to too many N: 1321 reads failed due to too short: 16468 reads with adapter trimmed: 50558 bases trimmed due to adapters: 3535293 Duplication rate (may be overestimated since this is SE data): 72.5533% JSON report: DabobBayWA_Q231.json HTML report: DabobBayWA_Q231.html fastp -i DabobBayWA_Q231.F.fq.gz -o DabobBayWA_Q231.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_Q231.json -h DabobBayWA_Q231.html fastp v0.20.0, time used: 24 seconds