WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCG Read1 before filtering: total reads: 3524531 total bases: 507532464 Q20 bases: 492399852(97.0184%) Q30 bases: 471908963(92.981%) Read1 after filtering: total reads: 3498724 total bases: 503777727 Q20 bases: 489245923(97.1154%) Q30 bases: 469059174(93.1084%) Filtering result: reads passed filter: 3498724 reads failed due to low quality: 6164 reads failed due to too many N: 2059 reads failed due to too short: 17584 reads with adapter trimmed: 23000 bases trimmed due to adapters: 1385947 Duplication rate (may be overestimated since this is SE data): 67.9244% JSON report: DabobBayWA_FG229.json HTML report: DabobBayWA_FG229.html fastp -i DabobBayWA_FG229.F.fq.gz -o DabobBayWA_FG229.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG229.json -h DabobBayWA_FG229.html fastp v0.20.0, time used: 37 seconds