WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
GAAGCAATACCGCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGCTGGAGAC

Read1 before filtering:
total reads: 2829673
total bases: 407472912
Q20 bases: 395870936(97.1527%)
Q30 bases: 380034141(93.2661%)

Read1 after filtering:
total reads: 2806912
total bases: 403322018
Q20 bases: 392216679(97.2465%)
Q30 bases: 376671251(93.3922%)

Filtering result:
reads passed filter: 2806912
reads failed due to low quality: 4660
reads failed due to too many N: 1650
reads failed due to too short: 16451
reads with adapter trimmed: 36661
bases trimmed due to adapters: 3240530

Duplication rate (may be overestimated since this is SE data): 69.5917%

JSON report: DabobBayWA_FG228.json
HTML report: DabobBayWA_FG228.html

fastp -i DabobBayWA_FG228.F.fq.gz -o DabobBayWA_FG228.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG228.json -h DabobBayWA_FG228.html 
fastp v0.20.0, time used: 28 seconds