WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... TGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTA Read1 before filtering: total reads: 2354208 total bases: 339005952 Q20 bases: 329352760(97.1525%) Q30 bases: 316115604(93.2478%) Read1 after filtering: total reads: 2344417 total bases: 337579794 Q20 bases: 328294411(97.2494%) Q30 bases: 315211892(93.374%) Filtering result: reads passed filter: 2344417 reads failed due to low quality: 4062 reads failed due to too many N: 1406 reads failed due to too short: 4323 reads with adapter trimmed: 6502 bases trimmed due to adapters: 637739 Duplication rate (may be overestimated since this is SE data): 69.7855% JSON report: DabobBayWA_FG226.json HTML report: DabobBayWA_FG226.html fastp -i DabobBayWA_FG226.F.fq.gz -o DabobBayWA_FG226.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG226.json -h DabobBayWA_FG226.html fastp v0.20.0, time used: 24 seconds