WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTC

Read1 before filtering:
total reads: 4785351
total bases: 689090544
Q20 bases: 669481772(97.1544%)
Q30 bases: 642583338(93.2509%)

Read1 after filtering:
total reads: 4758755
total bases: 684282739
Q20 bases: 665441248(97.2465%)
Q30 bases: 638926489(93.3717%)

Filtering result:
reads passed filter: 4758755
reads failed due to low quality: 7827
reads failed due to too many N: 2777
reads failed due to too short: 15992
reads with adapter trimmed: 36808
bases trimmed due to adapters: 3278232

Duplication rate (may be overestimated since this is SE data): 67.8395%

JSON report: DabobBayWA_FG224.json
HTML report: DabobBayWA_FG224.html

fastp -i DabobBayWA_FG224.F.fq.gz -o DabobBayWA_FG224.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG224.json -h DabobBayWA_FG224.html 
fastp v0.20.0, time used: 44 seconds