WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAAGAGAGATGAGATTGAGGCTG Read1 before filtering: total reads: 3677843 total bases: 529609392 Q20 bases: 514034653(97.0592%) Q30 bases: 493033054(93.0937%) Read1 after filtering: total reads: 3659045 total bases: 526892605 Q20 bases: 511915535(97.1575%) Q30 bases: 491173207(93.2207%) Filtering result: reads passed filter: 3659045 reads failed due to low quality: 6573 reads failed due to too many N: 2171 reads failed due to too short: 10054 reads with adapter trimmed: 11289 bases trimmed due to adapters: 1455233 Duplication rate (may be overestimated since this is SE data): 68.8065% JSON report: DabobBayWA_FG223.json HTML report: DabobBayWA_FG223.html fastp -i DabobBayWA_FG223.F.fq.gz -o DabobBayWA_FG223.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG223.json -h DabobBayWA_FG223.html fastp v0.20.0, time used: 33 seconds