WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAACAGTCGGGA Read1 before filtering: total reads: 4300589 total bases: 619284816 Q20 bases: 601521528(97.1316%) Q30 bases: 577284171(93.2179%) Read1 after filtering: total reads: 4259934 total bases: 613392739 Q20 bases: 596951995(97.3197%) Q30 bases: 573123379(93.435%) Filtering result: reads passed filter: 4259934 reads failed due to low quality: 11635 reads failed due to too many N: 5799 reads failed due to too short: 23221 reads with adapter trimmed: 27217 bases trimmed due to adapters: 3377948 Duplication rate (may be overestimated since this is SE data): 60.5445% JSON report: DabobBayWA_FG210.json HTML report: DabobBayWA_FG210.html fastp -i DabobBayWA_FG210.F.fq.gz -o DabobBayWA_FG210.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG210.json -h DabobBayWA_FG210.html fastp v0.20.0, time used: 45 seconds