WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... TTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAGC Read1 before filtering: total reads: 5033643 total bases: 724844592 Q20 bases: 704183741(97.1496%) Q30 bases: 676307493(93.3038%) Read1 after filtering: total reads: 5001526 total bases: 719863132 Q20 bases: 700756706(97.3458%) Q30 bases: 673318807(93.5343%) Filtering result: reads passed filter: 5001526 reads failed due to low quality: 14158 reads failed due to too many N: 6934 reads failed due to too short: 11025 reads with adapter trimmed: 26330 bases trimmed due to adapters: 1940665 Duplication rate (may be overestimated since this is SE data): 65.8428% JSON report: DabobBayWA_FG205.json HTML report: DabobBayWA_FG205.html fastp -i DabobBayWA_FG205.F.fq.gz -o DabobBayWA_FG205.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG205.json -h DabobBayWA_FG205.html fastp v0.20.0, time used: 47 seconds