WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... GCGGTAAAGTTAGACCAAACCATGAAACCAACATAAACATTATTGCCCGGCGTACGGGGA Read1 before filtering: total reads: 5472398 total bases: 788025312 Q20 bases: 765048490(97.0843%) Q30 bases: 733828088(93.1224%) Read1 after filtering: total reads: 5435668 total bases: 781796402 Q20 bases: 760493528(97.2751%) Q30 bases: 729780783(93.3467%) Filtering result: reads passed filter: 5435668 reads failed due to low quality: 14908 reads failed due to too many N: 7365 reads failed due to too short: 14457 reads with adapter trimmed: 37266 bases trimmed due to adapters: 3017785 Duplication rate (may be overestimated since this is SE data): 61.648% JSON report: DabobBayWA_FG203.json HTML report: DabobBayWA_FG203.html fastp -i DabobBayWA_FG203.F.fq.gz -o DabobBayWA_FG203.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG203.json -h DabobBayWA_FG203.html fastp v0.20.0, time used: 52 seconds