WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CTTAAACTTCTTAGACGAATCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGG Read1 before filtering: total reads: 4039122 total bases: 581633568 Q20 bases: 565315894(97.1945%) Q30 bases: 542788746(93.3214%) Read1 after filtering: total reads: 4007665 total bases: 576193994 Q20 bases: 561065176(97.3744%) Q30 bases: 538933145(93.5333%) Filtering result: reads passed filter: 4007665 reads failed due to low quality: 10087 reads failed due to too many N: 5319 reads failed due to too short: 16051 reads with adapter trimmed: 37011 bases trimmed due to adapters: 3216879 Duplication rate (may be overestimated since this is SE data): 65.7491% JSON report: DabobBayWA_FG201.json HTML report: DabobBayWA_FG201.html fastp -i DabobBayWA_FG201.F.fq.gz -o DabobBayWA_FG201.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG201.json -h DabobBayWA_FG201.html fastp v0.20.0, time used: 38 seconds