WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3419725
total bases: 492440400
Q20 bases: 472816244(96.0149%)
Q30 bases: 441321802(89.6193%)

Read1 after filtering:
total reads: 3412500
total bases: 491067596
Q20 bases: 472235406(96.1651%)
Q30 bases: 441012563(89.8069%)

Filtering result:
reads passed filter: 3412500
reads failed due to low quality: 6289
reads failed due to too many N: 936
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 45.5981%

JSON report: CypressIslandWA_FG149-SEQREP.json
HTML report: CypressIslandWA_FG149-SEQREP.html

fastp -i CypressIslandWA_FG149-SEQREP.F.fq.gz -o CypressIslandWA_FG149-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG149-SEQREP.json -h CypressIslandWA_FG149-SEQREP.html 
fastp v0.20.0, time used: 42 seconds