WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AAAAGTTTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAA

Read1 before filtering:
total reads: 3522056
total bases: 507176064
Q20 bases: 493585923(97.3204%)
Q30 bases: 474379869(93.5336%)

Read1 after filtering:
total reads: 3443087
total bases: 495039762
Q20 bases: 482224908(97.4113%)
Q30 bases: 463578737(93.6447%)

Filtering result:
reads passed filter: 3443087
reads failed due to low quality: 6475
reads failed due to too many N: 1950
reads failed due to too short: 70544
reads with adapter trimmed: 113000
bases trimmed due to adapters: 6406621

Duplication rate (may be overestimated since this is SE data): 65.3393%

JSON report: CypressIslandWA_FG148.json
HTML report: CypressIslandWA_FG148.html

fastp -i CypressIslandWA_FG148.F.fq.gz -o CypressIslandWA_FG148.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG148.json -h CypressIslandWA_FG148.html 
fastp v0.20.0, time used: 32 seconds