WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... CTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAA Read1 before filtering: total reads: 3639803 total bases: 524131632 Q20 bases: 509435760(97.1961%) Q30 bases: 489412205(93.3758%) Read1 after filtering: total reads: 3614626 total bases: 520463208 Q20 bases: 506290177(97.2768%) Q30 bases: 486524817(93.4792%) Filtering result: reads passed filter: 3614626 reads failed due to low quality: 5368 reads failed due to too many N: 2119 reads failed due to too short: 17690 reads with adapter trimmed: 23270 bases trimmed due to adapters: 2587400 Duplication rate (may be overestimated since this is SE data): 72.3736% JSON report: CypressIslandWA_FG147.json HTML report: CypressIslandWA_FG147.html fastp -i CypressIslandWA_FG147.F.fq.gz -o CypressIslandWA_FG147.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG147.json -h CypressIslandWA_FG147.html fastp v0.20.0, time used: 32 seconds