WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
CAGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCAT

Read1 before filtering:
total reads: 4295963
total bases: 618618672
Q20 bases: 602219479(97.3491%)
Q30 bases: 579198003(93.6276%)

Read1 after filtering:
total reads: 4273071
total bases: 613396387
Q20 bases: 597666037(97.4355%)
Q30 bases: 575014502(93.7427%)

Filtering result:
reads passed filter: 4273071
reads failed due to low quality: 6564
reads failed due to too many N: 2553
reads failed due to too short: 13775
reads with adapter trimmed: 59864
bases trimmed due to adapters: 3907610

Duplication rate (may be overestimated since this is SE data): 69.1798%

JSON report: CypressIslandWA_FG146.json
HTML report: CypressIslandWA_FG146.html

fastp -i CypressIslandWA_FG146.F.fq.gz -o CypressIslandWA_FG146.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG146.json -h CypressIslandWA_FG146.html 
fastp v0.20.0, time used: 38 seconds